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Cloning and Expression
Enzymes
AlwI
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AlwI
AlwI from New England Biolabs
Be the first to write a review!
Citations:
(13)
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Supplier Page from
New England Biolabs for
AlwI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AlwI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0513L
Quantity
2500 units
Concentration
2 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
GGATC(4/5)
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (13)
(1)
iMUT-seq: high-resolution DSB-induced mutation profiling reveals prevalent homologous-recombination dependent mutagenesis
Nat Commun
December 18, 2023
Aldo S. Bader, Martin Bushell
amplicon was then digested with the restriction enzymes Afl-II, Bts-I and Alw-I (
NEB
, #R0520, #R0667 and #
R0513
respectively) for 4 hours at 37 C with 300 rpm agitation in 1x
CutSmart buffer
(
NEB
). Following this,
(2)
In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila
STAR Protoc
December 16, 2022
Jocelyn L.Y. Tang, Robert Krautz, Oriol Llorà -Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. Brand
recombinant proteins" ,, 10 mM Tris-HCl (pH 8.0),N/A,N/A 100% ethanol,VWR,Cat#20821.330
AlwI
,
NEB
,Cat#
R0513L
Autoclaved dH2O,N/A,N/A "Cytiva Sera-mag Speedbeads (Carboxyl magnetic beads, 1 μM, 15 mL)",Fisher
(3)
A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medulla
eLife
August 30, 2022
Alokananda Ray, Xin Li
‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",
AlwI
,
New England Biolabs
,Catalog_#:
R0513S
,See section ‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",Quick
Ligation
kit,
New England Biolabs
(4)
BMAL1 moonlighting as a gatekeeper for LINE1 repression and cellular senescence in primates
Nucleic Acids Research
April 8, 2022
Chuqian Liang et al.
previously described (84). For adaptor trimming, the amplified DNA was sonicated and digested with
AlwI
(
R0513S
,
New England Biolabs
) enzyme. The DNA library was constructed using a NEBNext® Ultra™
DNA library prep
(5)
Microbes affect gut epithelial cell composition through immune-dependent regulation of intestinal stem cell differentiation
Cell Reports
March 29, 2022
Xi Liu, Peter Nagy, Alessandro Bonfini, Philip Houtz, Xiao-Li Bing, Xiaowei Yang, Nicolas Buchon
E220 sonicator (
Covaris
microtube
520045
). DamID adaptors were removed by
AlwI
(
New England Biolabs
R0513S
) overnight digestion. AMPure XP magnetic beads (
Beckman Coulter
A63880
) were used for cleanup for
sequencing
(6)
In vivo targeted DamID identifies CHD8 genomic targets in fetal mouse brain
iScience
November 19, 2021
A. Ayanna Wade, Jelle van den Ameele, Seth W. Cheetham, Rebecca Yakob, Andrea H. Brand, Alex S. Nord
were sonicated and prepped for
sequencing
. Sonicated samples were subjected to
AlwI
digestion (
NEB
,
R0513S
) to remove previously ligated adaptors and initial GATC sequences from fragments. A modified TruSeq
(7)
Stem cell niche organization in the Drosophila ovary requires the ECM component Perlecan
Current Biology
April 26, 2021
Alfonsa DÃaz-Torres, Alicia E. Rosales-Nieves, John R. Pearson, Carmen Santa-Cruz Mateos, Miriam MarÃn-Menguiano, Owen J. Marshall, Andrea H. Brand, Acaimo González-Reyes
T2694
DpnII
and
DpnII
buffer,
NEB
,Cat#
R0543S
Advantage 2 cDNA polymerase,Clontech,Cat# 639201
AlwI
,
NEB
,Cat#
R0513S
RNase A (DNase free),
Roche
,Cat#
11119915001
Qubit assay tubes
,
Invitrogen
,Cat#
Q32856
Qubit dsDNA HS assay kit
(8)
A KDM5–Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation
eLife
March 17, 2021
Hayden AM Hatch, Helen M Belalcazar, Owen J Marshall, Julie Secombe
,
New England BioLabs
,Cat# R0176, Other,
DpnI
I,New England BioLabs,Cat# R0543, Other,
AlwI
,
New England BioLabs
,Cat#
R0513
,
(9)
Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration
Cell research
February 1, 2021
Liang C, Liu Z, Song M, Li W, Wu Z, Wang Z, Wang Q, Wang S, Yan K, Sun L,
et al
.
previously described.58 For adapter trimming, the amplified DNA was sonicated and digested with
AlwI
(
R0513S
,
New England Biolabs
). The DNA library was constructed using a NEBNext Ultra
DNA Library Prep
Kit for
(10)
Ets21c Governs Tissue Renewal, Stress Tolerance, and Aging in the Drosophila Intestine
Cell Reports
June 4, 2019
Juliane Mundorf, Colin D. Donohoe, Colin D. McClure, Tony D. Southall, Mirka Uhlirova
,
New England Biolabs
,Cat. #R0543 MyTaq HS DNA Polymerase,
Bioline
,Cat. #
BIO-21112
AlwI
,
New England Biolabs
,Cat. #
R0513
Phusion High-Fidelity DNA Polymerase
,
New England Biolabs
,Cat. #M0530 Paraquat,Sigma-Aldrich,Cat. #856177 "1,4-Diazabicyclo[2.2.2]octane
(11)
Stem Cell Proliferation Is Kept in Check by the Chromatin Regulators Kismet/CHD7/CHD8 and Trr/MLL3/4
Developmental Cell
May 20, 2019
Louis Gervais et al.
B0202S
DpnI
,
NEB
,Cat#
R0176L
DpnII
,
NEB
,Cat#
R0543L
Sau3AI
,
NEB
,Cat#
R0169L
PCR buffer
MyTaq HS,
Bioline
,Cat#
BIO-21111
AlwI
,
NEB
,Cat#
R0513S
Klenow fragment,NEB,Cat# 210S
T4 polynucleotide kinase
,
NEB
,Cat#
M0201S
Elastase,Sigma,Cat# E0258-5MG ,, Critical
(12)
Cell-type-specific profiling of protein–DNA interactions without cell isolation using targeted DamID with next-generation sequencing
Nature Protocols
September 1, 2016
Owen J Marshall, Tony D Southall, Seth W Cheetham, Andrea H Brand
polymerase with hotstart is required to prevent primer dimer formation during
PCR
AlwI
(
NEB
, cat. no.
R0513S
) Nat Protoc. Author manuscript; available in PMC 2020 February 20. Marshall et al. Page 7 RNase
(13)
Cell type-specific profiling of protein-DNA interactions without cell isolation using Targeted DamID with next-generation sequencing
Nature Protocols
August 4, 2016
Maartje J Marshall, Tony D. Peric-Hupkes, Andrea H. van Steensel
polymerase with hotstart is required to prevent primer dimer formation during
PCR
AlwI
(
NEB
, cat. no.
R0513S
) RNase A (DNase-free) (
Roche
, cat. no.
11119915001
) diluted to 12.5μg/ml
Qubit assay tubes
(
Invitrogen
,
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AlwI from New England Biolabs
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